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Calcium Imaging of Auditory Organ Activity in Insects

Calcium Imaging of Auditory Organ Activity in Insects

Supervisor: Dr Berthold Hedwig

Project summary:

We recently developed a new method to inject permanent tracers and Ca-sensitive tracers into the peripheral nervous system of insects. This methods will be refined and used to load the peripheral hearing organ and the central axonal projections of auditory afferents and with a calcium sensitive tracer in insects like bushcrickets, crickets, moths or grasshoppers. After labelling the auditory pathway, the insect will be placed under an optical imaging system and sound stimuli will be used to explore the representation of acoustic signals in the hearing organ and in the central auditory neuropils by measuring the sound evoked changes in the optical Ca2+ signals.

References:

Pulver SR, Bayley TG, Taylor AL, Berni J, Bate M, Hedwig B (2015) Imaging fictive locomotor patterns in larval Drosphila. J Neurophysiology, http://jn.physiology.org/content/early /2015/08/21/jn.00731.2015

Baden, T and Hedwig B (2007). Neurite specific Ca2+ dynamics underlying sound processing in an auditory interneurone. J Neurobiol 67: 68-80.