Cell Biology
Torsten Krude
University Lecturer
Email: tk1@mole.bio.cam.ac.uk
Tel.: +44 (0)1223 330111
We are investigating the replication of chromosomal DNA in proliferating mammalian cells. Our research focuses on the mechanisms by which the initiation step of DNA replication is regulated, with a particular interest in the functional roles played by non-coding Y RNAs. Other research topics include the regulation of chromatin assembly during DNA synthesis and wider aspects of cell proliferation control.
We are addressing these topics experimentally by using cell biological and biochemical approaches, mainly employing cell-free systems derived from mammalian tissue culture cells.
Selected publications
- Collart, C., Christov, C.P., Smith, J.C., and Krude, T. (2011). The mid-blastula transition defines the onset of Y RNA-dependent DNA replication in Xenopus laevis. Mol Cell Biol 31, 3857-3870.
- Zhang, A.T., Langley, A.R., Christov, C.P., Kheir, E., Shafee, T., Gardiner, T.J., and Krude, T. (2011). Dynamic interaction of Y RNAs with chromatin and initiation proteins during human DNA replication. J Cell Sci 124, 2058-2069.
- Gardiner, T.J., Christov, C.P., Langley, A.R., and Krude, T. (2009). A conserved motif of vertebrate Y RNAs essential for chromosomal DNA replication. RNA 15, 1375-1385.
- Krude, T., Christov, C.P., Hyrien, O., and Marheineke, K. (2009). Y RNA functions at the initiation step of mammalian chromosomal DNA replication. J Cell Sci 122, 2836-2845.
- Christo, C. P., Gardiner, T. J., Szüts, D., and Krude, T. (2006). Functional requirement of non-coding Y RNAs for human chromosomal DNA replication. Mol Cell Biol 26, 6993-7004.
- Nabatiyan, A., Szüts, D., and Krude, T. (2006). Induction of CAF-1 expression in response to DNA strand breaks in quiescent human cells. Mol Cell Biol 26, 1839-1849.
- Nabatiyan, A., and Krude, T. (2004). Silencing of Chromatin Assembly Factor 1 in human cells leads to cell death and loss of chromatin assembly during DNA synthesis. Mol Cell Biol 24, 2853-2862.
- Krude, T. (2000). Initiation of human DNA replication in vitro using nuclei from cells arrested at an initiation-competent state. J Biol Chem 275, 13699-13707.
- Krude, T. , Jackman, M., Pines, J., and Laskey, R.A. (1997). Cyclin/Cdk-dependent initiation of DNA replication in a human cell-free system. Cell 88, 109-119.
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