Krude Group

Regulation of chromosomal DNA replication in human cells. Mechanisms of non-coding Y RNA function.

We are investigating the regulation of chromosomal DNA replication in eukaryotic cells. Our research focuses on the molecular mechanisms underlying the initiation step of DNA replication.

Current research topics include:

  • Investigating genome-wide specification and activation of human DNA replication origins
  • Characterising interactions between Y RNAs and chromatin during DNA replication

Key Publications: 

Krude, T., Bi, J., Doran, R., Jones, R.A., Smith J.C. (2025). Human DNA replication initiation sites are specified epigenetically by oxidation of 5-methyl-deoxycytidine. Nucleic Acids Res 53, gkaf362, 1-19.

Hyrien, O., Guilbaud, G. and Krude, T. (2025). The double life of mammalian DNA replication origins. Genes Dev 39, 304-324.

Guilbaud, G., Murat, P., Wilkes, H.S., Koch Lerner, L., Sale, J.E., and Krude, T. (2022). Determination of human DNA replication origin position and efficiency reveals principles of initiation zone organisation. Nucleic Acids Res 50, 10230-47.

Christov, C.P., Dingwell, K.S., Skehel, M., Wilkes, H.S., Sale, J.E., Smith, J.C., and Krude, T. (2018). A NuRD complex from Xenopus laevis eggs is essential for DNA replication during early embryogenesis. Cell Rep 22, 2265-2278.

Langley A.R., Gräf S., Smith J.C. and Krude T. (2016). Genome-wide identification and characterisation of human DNA replication origins by initiation site sequencing (ini-seq). Nucleic Acids Res 44, 10230-47.

Collart, C., Christov, C.P., Smith, J.C., and Krude, T. (2011). The mid-blastula transition defines the onset of Y RNA-dependent DNA replication in Xenopus laevis. Mol Cell Biol 31, 3857-3870.

Christov, C. P., Gardiner, T. J., Szüts, D., and Krude, T. (2006). Functional requirement of non-coding Y RNAs for human chromosomal DNA replication. Mol Cell Biol 26, 6993-7004.

Krude, T., Jackman, M., Pines, J., and Laskey, R.A. (1997). Cyclin/Cdk-dependent initiation of DNA replication in a human cell-free system. Cell 88, 109-119.

Full list of publications available via PubMed